BabyBioTM IDA columns for Immobilized Metal Ion Affinity Chromatography (IMAC) are ready-to-use for quick and easy purification of polyhistidine-tagged (His-tagged) proteins and other proteins with an affinity for metal ions. The columns are prepacked with pre-charged or uncharged WorkBeads 40 IDA resins. They are available in two column sizes, 1 ml and 5 ml. IMAC utilizes the affinity of histidine, cysteine and tryptophan amino acid side chains on the protein surface for binding to transition metal ions, such as Ni2+, Co2+, Cu2+ and Zn2+, which are immobilized via a metal chelating ligand on the chromatography resin.
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