BabyBio affimAb 5 mL x 1 45800105

BabyBio™ affimAb is 1 ml and 5 ml columns prepacked with the optimized alkali-stable resin WorkBeads affimAb designed for purification of monoclonal- and polyclonal antibodies. This resin has a superior base matrix in combination with an optimized alkali-stable protein A ligand. This results in high dynamic binding capacity also at short residence times, and stable capacity over multiple purification cycles with cleaning-in-place using 0.5 M NaOH.

BabyBio affimAb columns are convenient for efficient small-scale purifications and initial screening in process development.

• Top performance dynamic binding capacity also at short residence times
• Outstanding alkali stability with 0.5 M NaOH, extends the number of purification cycles
• Excellent purity, recovery, and reproducibility
• Negligible protein A leakage

Resin description

WorkBeads are agarose-based chromatographic resins manufactured by a proprietary method that results in porous beads with tight size distribution and very high mechanical stability. Agarose-based matrices have been successfully used for decades in biotechnology research from the laboratory to production scale, due to their exceptional compatibility with biomolecules including proteins, peptides, nucleic acids, and carbohydrates. WorkBeads resins are designed for separations requiring optimal capacity and purity.

The alkali stable recombinant protein A attached to the optimized base matrix is produced in E. coli under conditions free of components of animal origin and purified to high purity before coupling. This combination gives both high dynamic binding capacities for antibodies and the possibility for efficient cleaning-in-place with 0.5 M NaOH.

Column description

BabyBio column is made from biocompatible polypropylene which does not significantly interact with biomolecules. The top and bottom filters are made from low protein binding polyethylene. The ready-to-use BabyBio columns are delivered with a plugin the inlet, a cut-off outlet, and a cap for storage. The columns can be connected to a syringe, pump or chromatography system using finger-tight fittings (coned 10–32) for 1/16” o.d. tubing (standard HPLC PEEK tubing).