12 Cell Culture Inserts+12 Well Plate, 8 μm, PET Memberane, Non-Treated, Sterile, 12/pk, 48/cs, 724331

SKU: 724331

Manufacturer: Nest

Pack Size: 48

Cell Culture Inserts, PET Membrane

Individually packaged in peelable Tyvek film and Blister, Sterile

TC Treated Cat. No.	Non-Treated Cat. No.	Product Description	Pore Size (μm)	Membrane Transparence	/Pack	/Case
723121	723131	6 Cell Culture Inserts+6 Well Plate	0.4	Opaque	6	60
724121	724131	12 Cell Culture Inserts+12 Well Plate	0.4	Opaque	12	120
725121	725131	12 Cell Culture Inserts+24 Well Plate	0.4	Opaque	12	120
723021	723031	6 Cell Culture Inserts+6 Well Plate	3.0	Translucent	6	60
724021	724031	12 Cell Culture Inserts+12 Well Plate	3.0	Translucent	12	120
725021	725031	12 Cell Culture Inserts+24 Well Plate	3.0	Translucent	12	120
723421	723431	6 Cell Culture Inserts+6 Well Plate	1.0	Transparent	6	60
724421	724431	12 Cell Culture Inserts+12 Well Plate	1.0	Transparent	12	120
725421	725431	12 Cell Culture Inserts+24 Well Plate	1.0	Transparent	12	120
723321	723331	6 Cell Culture Inserts+6 Well Plate	8.0	Transparent	6	60
724321	724331	12 Cell Culture Inserts+12 Well Plate	8.0	Transparent	12	120
725321	725331	12 Cell Culture Inserts+24 Well Plate	8.0	Transparent	12	120

packing specification	Inserts/plate	Insert Diameter(mm)	Volume of Each Well (mL)	Inner Volume of Insert (mL)	Membrane Growth Area of Insert (cm²)
6	6 inserts/6well	24	2.6	1.5	4.67
12	12 inserts/6well	12	1.5	0.5	1.12
24	12 inserts/6well	6.5	0.6	0.1	0.33
100mm dish	1 inserts/6well	75	13	9	44

Pore Size of Membrane (μm)	Pore Density（well/cm²）
0.4	1x10⁸
1.0	2x10⁷
3.0	2x10⁶
5.0	4x10⁵
8.0	1x10⁵

Material:

Multiple well plate or dish is made from PS

Application:

Permeable supports, also known as cell culture inserts, are an essential tool for the study of both anchorage-dependent and independent cell lines.

This product produce a cell culture environment that closely resembles an in vivo state. Allow polarized cells to carry out metabolic activities in a more natural manner because the cells feed both apically and basolaterally.

Application: co-culture, epithelial cell polarity, migration, invasion, transport and permeability studies.

Product Feature:

·Innovative hanging design facilitates pipetting.

·Supplied in multiple well plates: 6-well, 12-well, 24well.

·PC membrane: low adsorption rate, reducing the loss of small molecular proteins and other compounds

·The PET membrane is not easy to be torn or curled, and it is easy to operate when taking out the chamber.

·Compatible with most solvents used for fixing and dyeing.

Attention：

• Attachment of cells grown on a permeable support (membrane) is sensitive to the initial seeding density, so the initial use should be inoculated with different density guarantee optimal growth;

•During the cultivation process, the liquid should be taken through the gap between the upper layer and the hole. When adding liquid, be careful and slow to avoid the damage of the membrane;

•Incubation of the permeable support in the medium prior to culturing the cells can improve cell attachment and distribution.

Guidelines for us：

• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight. • Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage. Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.• Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.

• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.

• Check the volume of the culture medium regularly and add fresh medium as needed.

• The cell layer can be directly fixed and stained in the nest using basic cytological methods. Note Avoid using solvents that dissolve the PC film.

• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage.

Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.

Attribute name	Attribute value
Pack Size	48
Inserts Packing	12
Wells	12-well
Pore Size	8.0um
Media	PET (Polyester)
Type	Non-treated

100% NZ Owned Company

Phone: 0800 1 289 266

Email: sales@microanalytix.co.nz

12 Cell Culture Inserts+12 Well Plate, 8 μm, PET Memberane, Non-Treated, Sterile, 12/pk, 48/cs, 724331

Information

Customer service

My account

Contact Us

100% NZ Owned Company

Phone: 0800 1 289 266

Email: sales@microanalytix.co.nz

hello, customer

12 Cell Culture Inserts+12 Well Plate, 8 μm, PET Memberane, Non-Treated, Sterile, 12/pk, 48/cs, 724331

Information

Customer service

My account

Contact Us